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microsatellite analysis (Animalia)
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Knowledge graph centered on microsatellite analysis (Animalia) with 40 nodes and 118 connections. Top connected: Unknown, Marmota flaviventris, neighborhood effects, population dynamics, Sage grouse.
Description
Pooled whole genome sequencing approach using DNA from multiple individuals per population to identify SNPs associated with environmental variables and population differentiation patterns.
Typical Equipment
- collection containers
- ethanol
- CTAB solution
- Qubit
- Illumina HiSeq3000
- FASTQC
- TRIMMOMATIC
- BWA-MEM
- SAMTOOLS
- GATK
Output Measurements
- SNP frequencies
- FST values
- population genetic statistics
Papers Using This Protocol (4)
article
Evolution and genomic basis of the plant penetrating ovipositor: a key morphological trait in herbivorous Drosophilidae
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Cold adaptation drives population genomic divergence in the ecological specialist, <i> Drosophila montana </i>
article
Phylogeographic patterns in <i>Drosophila montana</i>
article