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PCR-based complete viral genome recovery (Sciuridae)
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Description
Method using abutting primers designed from contigs to amplify and recover complete circular viral genomes, followed by cloning and Sanger sequencing for verification.
Typical Equipment
- Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, USA)
- pJET 1.2 vector
- Escherichia coli DH5-alpha competent cells
Output Measurements
- complete viral genome sequences