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PoolSeq vs individual short-read polishing comparison (Pieridae)

Subcategory: genome polishing
Papers: 1 | Mentions: 1

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Knowledge graph centered on PoolSeq vs individual short-read polishing comparison (Pieridae) with 31 nodes and 109 connections. Top connected: Ligusticum porteri, Lepidoptera, Papilio, non-native plants, Pieris napi.

Description

Comparative analysis of genome assembly improvement using pooled vs individual Illumina short reads for polishing long-read assemblies, assessed using BUSCO completeness metrics.

Typical Equipment

  • Illumina HiSeq platform
  • Pilon (v.1.23)
  • SeqKit
  • NextGenMap

Output Measurements

  • BUSCO scores
  • assembly completeness
  • ortholog hit ratios

Papers Using This Protocol (1)

article

The genome of the Margined White butterfly (Pieris macdunnoughii): sex chromosome insights and the power of polishing with PoolSeq data

2021Genome Biology and Evolutionintroducing

Species Studied With This Protocol

Lepidoptera

1 paper

native fish

1 paper

non-native plants

1 paper

Arthropoda

1 paper

native fish species

1 paper

Ligusticum porteri

Apiaceae1 paper

native fishes

1 paper

Pieris napi

Pieridae1 paper

Pieris macdunnoughii

Pieridae1 paper

Alliaria petiolata

Brassicaceae1 paper