RNA sequencing (Sciuridae)

Local Knowledge Graph (38 entities)

Knowledge graph centered on RNA sequencing (Sciuridae) with 38 nodes and 132 connections. Top connected: Marmota flaviventris, Atriplex canescens, mark-recapture (Sciuridae), Bacteria, Zonotrichia leucophrys.

Description

RNA extraction from blood samples, library preparation, and high-throughput sequencing on Illumina platforms followed by read mapping and differential gene expression analysis using mixed models.

Typical Equipment

• TruSeq Library Prep Kit v2
• Illumina HiSeq2000
• Illumina HiSeq4000
• TopHat2
• HT-Seq

Output Measurements

• gene expression levels
• transcript counts
• differential expression statistics

Papers Using This Protocol (2)

Species Studied With This Protocol