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RNA-seq differential expression analysis (Sciuridae)
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Description
Bioinformatics pipeline for processing RNA-seq reads including quality control, mapping to reference genome, and statistical analysis of differential gene expression using mixed models.
Typical Equipment
- Trim Galore
- TopHat2 v2.1.0
- EMMERML
- LIMMA package
- R version 3.3.1
Output Measurements
- differentially expressed genes
- fold changes
- q-values