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Comparative chromosome painting (Brassicaceae)

Subcategory: cytogenetic
Standard reference: Lysak and Mandáková (2013)
Papers: 2 | Mentions: 3

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Knowledge graph centered on Comparative chromosome painting (Brassicaceae) with 38 nodes and 126 connections. Top connected: Unknown, not mentioned, Atriplex canescens, Asteraceae, Bacteria.

Description

Chromosome-specific BAC contigs of Arabidopsis thaliana were used as painting probes to analyze mitotic and meiotic chromosomes of C. cordifolia. Fluorescent signals were detected and analyzed to determine chromosome structure and evolutionary relationships.

Typical Equipment

  • RNase
  • pepsin
  • biotin-dUTP
  • digoxigenin-dUTP
  • Cy3-dUTP
  • DAPI
  • Olympus BX-51 microscope

Output Measurements

  • chromosome structure
  • translocation events
  • karyotype composition