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Transgenic functional complementation assay (Brassicaceae)
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Knowledge graph centered on Transgenic functional complementation assay (Brassicaceae) with 24 nodes and 95 connections. Top connected: Ligusticum porteri, Arabidopsis, Boechera stricta, Boechera, Arabidopsis thaliana.
Description
Creation and analysis of transgenic Arabidopsis lines expressing candidate genes from study species to test gene function and allelic effects. Uses heterologous expression system to isolate gene effects.
Typical Equipment
- transformation vectors
- plant growth chambers
- HPLC systems
Output Measurements
- transgenic plant glucosinolate levels
- gene expression patterns
- chemical phenotypes