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RNA-seq (Drosophilidae)
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Description
Illumina GAIIx RNA sequencing of larval samples from different plant treatments followed by differential expression analysis to identify genes responsive to plant chemical defenses.
Typical Equipment
- Qiagen Tissue Lysis Buffer
- gDNA Eliminator spin column
- Agilent 2100 Bioanalyzer
- RNA Nano Chip
- Illumina mRNA sequencing sample prep kit
- Illumina GAIIx sequencer
- AbySS version 1.2.1 software
Output Measurements
- gene expression levels
- differentially expressed genes