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COI DNA barcoding (Baetidae)
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Knowledge graph centered on COI DNA barcoding (Baetidae) with 35 nodes and 127 connections. Top connected: Rushes, aquatic insects, Ambystoma, phenotypic plasticity, Ambystoma tigrinum.
Description
DNA extraction using modified Chelex protocols followed by PCR amplification and sequencing of the mitochondrial cytochrome oxidase I gene to assess genetic variation within and among mayfly populations.
Typical Equipment
- Eppendorf tubes
- chelex solution
- proteinase K
- PCR equipment
- LCO1490 primer
- HCO2198 primer
- Applied Biosystems 377 automated sequencer
Output Measurements
- DNA sequences
- haplotype frequencies
- sequence divergence