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Controlled freezing stress treatment (Liliaceae)
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Description
In situ freezing treatment using liquid nitrogen and heat exchanger to gradually cool plant tissues to -30°C. Designed to simulate natural freezing events while allowing controlled temperature ramping and monitoring.
Typical Equipment
- cylindrical styrofoam chambers
- 70 L Dewar flask
- coiled copper tubing heat exchanger
- digital microprocessor thermometer
Output Measurements
- leaf temperature
- air temperature
- freezing stress response