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Bacterial culture isolation and maintenance from plant phyllosphere (Pseudomonadaceae)
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Knowledge graph centered on Bacterial culture isolation and maintenance from plant phyllosphere (Pseudomonadaceae) with 14 nodes and 32 connections. Top connected: Bacteria, Cardamine cordifolia, plant-microbial coupling, Pseudomonas, genome annotation.
Description
Protocol for isolating and maintaining pure bacterial cultures from plant leaf surfaces through surface sterilization, plating on selective media, and cryopreservation in glycerol stocks.
Typical Equipment
- King's B (KB) agar plates
- KB broth
- shaking incubator
- freezer
Output Measurements
- pure bacterial cultures
- frozen stock cultures