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Microbial dormancy molecular analysis
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Knowledge graph centered on Microbial dormancy molecular analysis with 11 nodes and 23 connections. Top connected: Bacteria, elevational gradients, drive-point piezometer groundwater sampling, 16S rRNA gene sequencing, viral operational taxonomic units.
Description
DNA and RNA co-extraction followed by cDNA synthesis and next-generation sequencing to assess relative abundance of active versus dormant microbial operational taxonomic units. Uses ratio of rRNA to rDNA recovery to determine dormancy levels.
Typical Equipment
- MoBio DNeasy kits
- RNeasy kits
- random hexamers
- SMARTscribe Reverse Transcriptase Kit
- next-generation sequencing platform
Output Measurements
- relative abundance of active OTUs
- relative abundance of dormant OTUs
- rRNA:rDNA ratios