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16S rRNA qPCR detection of skin mites (Vespertilionidae)

Subcategory: molecular
Standard reference: Ferreira et al. [5]
Papers: 1 | Mentions: 1

Local Knowledge Graph (26 entities)

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Knowledge graph centered on 16S rRNA qPCR detection of skin mites (Vespertilionidae) with 26 nodes and 90 connections. Top connected: Marmota flaviventris, Lepidoptera, Odocoileus, fish and wildlife, Detection, prevalence and phylogenetic relationshi.

Description

Real-time PCR using universal 16S primers to detect mite DNA in mammalian hair samples, with melting curve analysis for specificity assessment.

Typical Equipment

  • Roche LightCycler 480 Real-Time PCR System
  • FastStart Universal SYBR Green Master

Output Measurements

  • mite DNA presence/absence
  • melting temperature
  • amplification cycles

Papers Using This Protocol (1)

article

Detection, prevalence and phylogenetic relationships of <i>Demodex</i> spp and further skin prostigmata mites (Acari, Arachnida) in wild and domestic mammals

2016PLoS ONEusing

Species Studied With This Protocol

Odocoileus

Cervidae1 paper

white-tailed deer

1 paper

Canis lupus

Canidae1 paper

Eptesicus fuscus

1 paper

Lepidoptera

1 paper

Marmota flaviventris

Sciuridae1 paper

Homo sapiens

Hominidae1 paper

Arthropoda

1 paper

fish and wildlife

1 paper

Papilionidae

Papilionidae1 paper