Microsatellite enrichment and primer development (Polemoniaceae)

Local Knowledge Graph (16 entities)

Knowledge graph centered on Microsatellite enrichment and primer development (Polemoniaceae) with 16 nodes and 43 connections. Top connected: Ipomopsis, I. aggregata, Ipomopsis aggregata, Ipomopsis aggregata ssp. aggregata, I. tenuituba.

Description

Development of microsatellite primers through genomic library construction, enrichment, cloning, sequencing, and primer design for population genetic studies.

Typical Equipment

• RsaI endonuclease
• biotinylated oligonucleotides
• TOPO TA Cloning vector
• Escherichia coli TOP10 competent cells
• Primer3 software

Output Measurements

• microsatellite primer sequences
• allele sizes
• polymorphism metrics

Papers Using This Protocol (1)

Species Studied With This Protocol