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DAB staining (Drosophilidae)

Subcategory: plant defense response
Standard reference: Clarke 2009
Papers: 1 | Mentions: 1

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Knowledge graph centered on DAB staining (Drosophilidae) with 37 nodes and 121 connections. Top connected: Unknown, Ligusticum porteri, Lepidoptera, Arabidopsis, Boechera.

Description

Histochemical staining using DAB for hydrogen peroxide and NBT for superoxide detection in plant tissues following herbivore attack. Reveals spatial patterns of oxidative stress responses around feeding damage sites.

Typical Equipment

  • 3,3'-diaminobenzidine tetrahydrochloride (DAB)
  • nitro blue tetrazolium (NBT)
  • microscope

Output Measurements

  • H₂O₂ localization
  • superoxide localization

Papers Using This Protocol (1)

article

Mining the plant-insect interface with a leafmining <i>Drosophila</i> of <i>Arabidopsis</i>

2011Molecular Ecologyusing

Species Studied With This Protocol

Cardamine cordifolia

Brassicaceae1 paper

Brassica oleracea

Brassicaceae1 paper

Drosophila virilis

Drosophilidae1 paper

Lepidoptera

1 paper

browse

1 paper

woody stems

1 paper

Ligusticum porteri

Apiaceae1 paper

Brassica

Brassicaceae1 paper

Nicotiana

Solanaceae1 paper

Pisum sativum

1 paper