← Back to Protocolslaboratory
Bacterial endophyte isolation from crucifer leaves (Brassicaceae)
Local Knowledge Graph (18 entities)
Loading graph...
Knowledge graph centered on Bacterial endophyte isolation from crucifer leaves (Brassicaceae) with 18 nodes and 83 connections. Top connected: Unknown, Ligusticum porteri, Bacteria, Arabidopsis, Arabidopsis thaliana.
Description
Surface sterilization of plant leaves followed by tissue plating on selective media to isolate bacterial endophytes and assess their growth characteristics and pathogenicity.
Typical Equipment
- cork borer
- Kings B media agar
- 70% ethanol
- blunt syringe
- solid agar plates
Output Measurements
- bacterial colony counts
- growth rate
- virulence symptoms