PCR amplification of bacterial endosymbionts (Aphididae)

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Knowledge graph centered on PCR amplification of bacterial endosymbionts (Aphididae) with 31 nodes and 109 connections. Top connected: Unknown, Bacteria, Artemisia, A. tridentata, Artemisia tridentata.

Description

DNA extraction from aphids followed by PCR amplification using specific primers for H. defensa and B. aphidicola, with gel electrophoresis visualization.

Typical Equipment

• PCR thermal cycler
• agarose gel electrophoresis
• EtBr staining

Output Measurements

• presence/absence of H. defensa
• presence/absence of B. aphidicola

Papers Using This Protocol (1)

Species Studied With This Protocol