skeletochronology (Ambystomatidae)

Local Knowledge Graph (18 entities)

Knowledge graph centered on skeletochronology (Ambystomatidae) with 18 nodes and 54 connections. Top connected: Ambystoma, Ambystoma tigrinum, Ambystoma tigrinum nebulosm, tiger salamander, Ambystoma mavortium.

Description

Standard skeletochronology protocol involving tissue fixation, decalcification, cryostat sectioning, histological staining, and LAG counting to determine age in amphibians.

Typical Equipment

• 70% ethanol
• 3% nitric acid
• cryostat (HM 505 E)
• Harris' haematoxylin stain
• phase-contrast microscope

Output Measurements

• estimated age in years
• LAG count

Papers Using This Protocol (1)

Species Studied With This Protocol