Protocols

Microscopic assessment of arbuscular mycorrhizal fungal colonization and other fungal colonization of plant roots using crosshair intercept method and grid line-intercept method for soil fungi.

Hierarchical Bayesian models predicting vital rates from individual and neighborhood characteristics, with population-level random effects and model selection via WAIC.

Multivariate analysis technique to quantify community responses over time. Treatment weights indicate response magnitude and species weights show individual taxa responses.

Standard chloroform fumigation direct extraction technique for measuring soil microbial biomass, combined with soil chemistry analysis and enzyme activity assays.

Comprehensive search of Web of Science databases for manipulative experiments on terrestrial carbon cycling responses to global change drivers, followed by systematic screening, data extraction, and q...

Laboratory analysis of plant samples for nitrogen content using elemental CHNSO analyzer and water content determination through oven-drying weight loss.

Field identification of lichen species using chemical spot tests with potassium hydroxide, morphological examination with hand lens, and comparison to field guide keys.

Calculation of Shannon diversity index (ln type) and Simpson dominance index (1-D) from species count data using online biodiversity calculator. Standard ecological diversity metrics for comparing com...

Estimation of invertebrate biomass using established taxon-specific length-mass relationships and scaling to aerial measures.