Protocols

A 2×2 factorial experiment combining passive warming using open-top chambers with dominant plant species removal across elevational gradients at multiple global mountain sites to test interactive effe...

29-year experimental warming using overhead infrared radiators to increase soil temperature by 2°C in subalpine grassland plots. Heat flux of 22 W/m² applied continuously from 1991-2019.

Factorial experiment crossing nitrogen addition treatments with dominant species removal treatments in replicated plots to test community responses to multiple global change drivers. Includes both sel...

Factorial greenhouse experiment testing effects of soil inoculum source and sterilization on alpine grass growth, traits, and fungal colonization. Plants grown in standardized sand-inoculum mixtures w...

A controlled field experiment transplanting grass species across elevational gradients with manipulated fungal soil communities to test effects of plant-fungi mismatches on plant performance during cl...

Field experiment testing seed germination of different genotypes/treatments in natural microhabitats of related species to assess niche requirements and differentiation.

Seeds were treated with Benomyl fungicide at 4°C to remove endophytic fungi, creating endophyte-free plants for comparison with naturally endophyte-positive and endophyte-negative plants.