Protocols
11 protocols
instar staging
Standardized measurements of wing length and dried body mass components under stereomicroscope to quantify adult fitness proxies and assess elevation effects on body size.
Beaver pond habitat characterization
Multi-parameter assessment of beaver pond physical characteristics including area, depth, flow velocity, and beaver activity indicators to control for habitat differences in community analyses.
Stream quality assessment
Comprehensive measurement of stream physical and chemical parameters including substrate characteristics, water quality, and algal biomass. Provides environmental context for species distribution patt...
oven-dry mass measurement
Standardized measurement of individual larval dry mass using precision microbalance after oven-drying at controlled temperature. Applied to assess growth and biomass responses to experimental treatmen...
Multi-parameter water quality monitoring (Amphibia)
In-situ measurement of temperature, pH, dissolved oxygen, and barometric pressure at multiple points around each pond during peak photosynthetic activity. Provides standardized water chemistry profile...
Salamander biofluorescence photography under blue excitation (Amphibia)
Standardized photography protocol using blue light excitation to capture biofluorescence in salamanders under controlled conditions with specific camera settings and positioning.
body condition index (Ambystomatidae)
Calculation of body condition metrics (mass/SVL³) and derived fitness proxies including condition-weighted reproductive opportunities for males and estimated egg production for females based on body s...
Ceramic tile algal biomass estimation
Use of ceramic tiles as standardized substrates for measuring benthic algal biomass via chlorophyll-a extraction and fluorometry. Includes grazed vs ungrazed comparisons to assess herbivory effects.
Ecosystem nutrient uptake measurement (Amphibia)
Nutrient addition experiments in benthic substrates and sedge biofilms to measure ecosystem demand through exponential decay models. Measures uptake capacity of different pond compartments.
Grid-based topographic measurement
Systematic measurement of slope and aspect at grid cell centers using compass and clinometer with clipboard to minimize micro-scale surface variation effects.
Stream Solute Workshop method
Tracer Additions for Spiraling Curve Characterization method involving simultaneous injection of water tracer and nutrients with downstream breakthrough curve monitoring to quantify reach-scale nutrie...