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Protocols

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6 protocols

samplingstandardized

quadrat sampling method

Measurement of plant species-specific cover, identification of focal species, and collection of plant biomass and functional traits including specific leaf area and leaf dry matter content.

vegetation monitoring48 papersmeasurement tools for biomass
samplingstandardized

Loss-on-ignition method

Systematic excavation of 1-meter soil pits with depth-stratified sampling at 10 cm intervals for soil organic carbon, nitrogen, and dissolved organic carbon analysis. Includes soil profile description...

biogeochemical16 papersshovels, D5600 Nikon camera...
samplingstandardized

Alpine soil inocula collection and preparation

Collection of soil inocula from resident (high elevation) and novel (low elevation) sites across elevation gradients in mountain ecosystems, with subsequent sterilization treatments to isolate microbi...

soil microbial community8 papers8.5% sodium hypochlorite bleach-sterilized trowel, ethanol-sterilized 2 mm grade sieve...
sampling

Species-area curve analysis

Systematic sampling of lichen cover and species composition along fixed 1-meter transects on rock surfaces, with digital photography and image analysis for precise measurement of lichen thallus length...

vegetation survey3 papersmeter stick, iPhone 6s...
sampling

Elevational gradient soil and seed sampling (Fabaceae)

Systematic collection of soils and seeds across elevational gradients spanning current and projected future species ranges to capture natural variation in soil microbial communities and plant populati...

field collection2 paperssoil coring tools
samplingstandardized

percent cover estimation

Long-term monitoring of tundra plant communities using standardized sampling within permanently marked study areas, employing various abundance quantification methods including point frame hits, visua...

vegetation monitoring2 papersPoint frames, visual estimation methods...