Protocols
13 protocols
benthic sampling
Plastic pan traps with soapy water deployed along stream transects to capture terrestrial invertebrates falling into or near the river. Traps are pooled by transect and processed for taxonomic identif...
mark-recapture (Ambystomatidae)
Annual capture-recapture surveys over 24 years to track individual survival, growth, reproduction and developmental pathways in a polyphenic salamander population. Combines individual marking with det...
slit emergence traps
Systematic collection of water samples from ponds across a hydroperiod gradient for analysis of nitrogen and phosphorus compounds. Samples collected at multiple time points to capture temporal variati...
Chlorophyll-a extraction and fluorometric analysis
Collection of nitrogen samples via syringe filtration and chlorophyll samples via algae collection from submerged rocks, with spectrophotometric analysis normalized by substrate area.
gastric-lavage (Ambystomatidae)
Modified gastric-lavage technique to obtain stomach contents for prey identification, enumeration, and biomass/caloric calculations using published conversion factors.
d-frame net sampling
Standardized sampling of large and small benthic invertebrates using D-net sweeps and benthic cores, with zooplankton sampling using mesh nets. Animals identified to species or family level for commun...
Ash-free dry mass
Collection and processing of biofilm samples from natural and artificial substrates to quantify biomass, algal content, and nutrient content through AFDM, chlorophyll-a, and phosphorus analysis.
CDC light trap sampling (Culicidae)
Opportunistic collection of adult and larval mosquitoes across elevational gradients using standardized collection techniques. Adults collected during blood-feeding attempts, larvae sampled from diver...
removal method electrofishing
Three-pass electrofishing to estimate fish densities and species composition in stream reaches for characterizing predator presence and abundance.
Adult mosquito collection and identification (Culicidae)
Collection of adult mosquitoes during blood-seeking behavior and other activities to complement larval surveys and confirm species identifications.
Fish mucus extraction and processing (Baetidae)
Method for collecting fish skin mucus by gentle rubbing in plastic bags followed by freeze-drying and chemical characterization of mucus components including glycosaminoglycans, hexosamine, and uronic...
Systematic grid mapping of sagebrush populations (Asteraceae)
Complete census of sagebrush individuals within systematic 16m × 16m grids divided into 1m² cells, with measurement of plant dimensions for size estimation.
Tile-based didymo sampling and enumeration
Standardized substrate tiles deployed in stream channels for algae colonization, followed by systematic scraping, filtering, and microscopic enumeration of didymo cells.