Protocols

Controlled behavioral experiments using salamanders in tanks with traps at either end to test preferences for different light stimuli and biofluorescent vs. non-biofluorescent mates. Salamanders place...

Standardized substrate tiles deployed in stream channels for algae colonization, followed by systematic scraping, filtering, and microscopic enumeration of didymo cells.

Cellulose acetate electrophoresis to analyze genetic variation at enzyme loci in mayfly populations, involving initial enzyme screening, locus selection, and final allele frequency determination acros...

Classification of ponds into permanent, semi-permanent, and temporary categories based on historical drying patterns and hydroperiod duration from long-term data records.

Statistical analysis comparing observed vs. expected species co-occurrence patterns using probabilistic models to identify positive, negative, or random associations between species pairs.