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microsatellite DNA analysis (Formicidae)

Subcategory: molecular genetics
Papers: 1 | Mentions: 1

Local Knowledge Graph (29 entities)

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Knowledge graph centered on microsatellite DNA analysis (Formicidae) with 29 nodes and 105 connections. Top connected: Hummingbird, Spruce, Selasphorus platycercus, Peromyscus, Selasphorus rufus.

Description

DNA extraction from ant specimens followed by PCR amplification of microsatellite loci and relatedness estimation using maximum likelihood methods based on population allele frequencies.

Typical Equipment

  • microcentrifuge tubes
  • hand drill
  • lysis buffer
  • PCR reagents
  • Techne Laboratories thermal cycler
  • polyacrylamide gels

Output Measurements

  • relatedness coefficients
  • allele frequencies
  • genetic diversity estimates

Papers Using This Protocol (1)

student_paper

Assessing the Impact that the Keystone Species, the Red Naped Sapsucker (<i> Sphyrapicus nuchalis </i>), has on the Community of Species in the East River

2022using

Species Studied With This Protocol

Spruce

1 paper

Phellinus Tremulae

1 paper

Peromyscus

Cricetidae1 paper

Selasphorus rufus

Trochilidae1 paper

broad-tailed hummingbirds

1 paper

Zapus princeps

Dipodidae1 paper

Hummingbird

1 paper

Tamiasciurus hudsonicus

Sciuridae1 paper

Tachycineta bicolor

Hirundinidae1 paper

flora and fauna

1 paper