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Bee gut bacterial isolation and genome sequencing (Orbaceae)

Subcategory: microbial isolation
Standard reference: Kwong WK, Moran NA. 2016. Gut microbial communities of social bees. Nat Rev Microbiol 14:374–384
Papers: 1 | Mentions: 1

Local Knowledge Graph (28 entities)

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Knowledge graph centered on Bee gut bacterial isolation and genome sequencing (Orbaceae) with 28 nodes and 103 connections. Top connected: Bombus, Bombus terrestris, Bombus appositus, Bombus impatiens, B. appositus.

Description

Isolation of pure bacterial cultures from bee gut homogenates followed by whole genome sequencing using Illumina MiSeq and PacBio platforms with subsequent assembly and annotation.

Typical Equipment

  • 10 mM MgSO4
  • heart infusion agar
  • Illumina MiSeq
  • PacBio RS II

Output Measurements

  • bacterial isolate cultures
  • genome sequences
  • phylogenetic relationships

Papers Using This Protocol (1)

article

Metabolism of toxic sugars by the bee gut symbiont <i>Gilliamella apicola</i>

2017mBiousing

Species Studied With This Protocol

Bacteria

1 paper

Escherichia coli

Enterobacteriaceae1 paper

Bombus

Apidae1 paper

Apis mellifera

Apidae1 paper

Bombus appositus

Apidae1 paper

B. vosnesenskii

1 paper

Bombus vosnesenskii

Apidae1 paper

B. rufocinctus

1 paper

Proteobacteria

1 paper

Firmicutes

1 paper