← Back to Protocolsanalyticalstandardized
Microsatellite genotyping (Polemoniaceae)
Local Knowledge Graph (39 entities)
Loading graph...
Knowledge graph centered on Microsatellite genotyping (Polemoniaceae) with 39 nodes and 138 connections. Top connected: Unknown, Hummingbird, Ipomopsis, I. aggregata, Ipomopsis aggregata.
Description
PCR amplification of microsatellite loci followed by capillary electrophoresis for allele sizing and polymorphism assessment.
Typical Equipment
- Eppendorf Mastercycler thermocyclers
- ABI 3730xl sequencer
- ROX 500 size standard
- GeneMarker software
Output Measurements
- allele sizes
- heterozygosity
- number of alleles per locus
Papers Using This Protocol (3)
article
Microsatellite loci in <i>Ipomopsis aggregata</i> (Polemoniaceae) and cross-species applicability for ecological genetics studies
article
Genetic and morphological patterns show variation in frequency of hybrids between <i>Ipomopsis</i> (Polemoneaceae) zones of sympatry
article