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Standard plant histology (Polemoniaceae)
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Description
Standard plant histological protocol involving glutaraldehyde fixation, resin embedding, serial sectioning at 1.5 μm, and multiple staining techniques including toluidine blue for general structure and specialized stains for cell wall components.
Typical Equipment
- 3% glutaraldehyde
- 0.1 M sodium cacodylate buffer
- ethanol series
- Spurr's resin
- microtome
- toluidine blue
- ether aniline blue
- phloroglucinol
- ruthenium red
Output Measurements
- cellular structure
- cell wall composition
- tissue organization