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Bumble bee pathogen microscopy survey (Apidae)

Subcategory: parasite detection
Papers: 1 | Mentions: 1

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Knowledge graph centered on Bumble bee pathogen microscopy survey (Apidae) with 27 nodes and 101 connections. Top connected: Unknown, Bombus, Bombus terrestris, Bombus appositus, Bombus impatiens.

Description

Dissection of bumble bees followed by microscopic examination of gut and fat body tissues for protozoan parasites using Giemsa staining. Quantifies pathogen intensity by counting cells in standardized fields of view.

Typical Equipment

  • dissection tools
  • microscope slides
  • methanol
  • Giemsa solution
  • phosphate buffer
  • water
  • compound microscope

Output Measurements

  • pathogen presence/absence
  • pathogen intensity (cell counts)
  • infection prevalence