Protocols

Fluorometric analysis of ammonia in water samples using working reagent that produces fluorescent compound when reacting with ammonia. Uses quadruplicate sampling and background correction.

Standard colorimetric method for measuring soluble reactive phosphorus and total dissolved phosphorus in water samples using spectrophotometry. Total dissolved phosphorus samples are first digested wi...

Collection and integration of climate variables from multiple databases including temperature, solar radiation, snow metrics, and topographic factors to characterize environmental conditions at study ...

DNA extraction using modified Chelex protocols followed by PCR amplification and sequencing of the mitochondrial cytochrome oxidase I gene to assess genetic variation within and among mayfly populatio...

Systematic search and compilation of published records of D. geminata blooms with associated soluble reactive phosphorus measurements to characterize global patterns.

Modified gut content analysis method that estimates feeding intensity by counting the proportion of abdominal segments containing food rather than identifying specific prey items.

Magnesium hydroxide co-precipitation method using Sargasso Sea water to concentrate low-level soluble reactive phosphorus samples 70-fold prior to colorimetric analysis.

Cellulose acetate electrophoresis to analyze genetic variation at enzyme loci in mayfly populations, involving initial enzyme screening, locus selection, and final allele frequency determination acros...

Statistical analysis comparing observed vs. expected species co-occurrence patterns using probabilistic models to identify positive, negative, or random associations between species pairs.