Protocols

Multi-site common garden experiment using controlled crosses to partition genetic and environmental variance in vegetative and floral traits across a hybrid zone. Seeds from multiple genetic families ...

Paired experimental design comparing floral development and longevity between plants receiving daily road dust applications versus undusted controls, with detailed tracking of flower developmental sta...

A controlled field experiment testing the effects of nitrogen addition and reduction on plant community composition over 8 years. Annual percent cover monitoring tracked responses of invasive and nati...

Controlled experiment using artificial flowers with discrete bullseye phenotypes exposed to UV-present vs UV-absent treatments to test UV-mediated selection on floral traits. Pollen viability measured...

Experimental clipping treatment designed to reduce plant photosynthetic capacity by approximately 50% through removal of stems. Used to simulate herbivory stress and examine effects on mycorrhizal ass...

Plants were subjected to progressive water stress by withholding irrigation to create a gradient of soil moisture conditions. This experimental design allowed examination of plant responses across a r...

Controlled application of synthetic pinene enantiomers via cotton-filled microcentrifuge tube emitters to test effects of specific volatile compounds on insect oviposition behavior. Treatments applied...

Production of F2 hybrid generations with alternate cytoplasmic backgrounds through controlled hand-pollination crosses. Allows testing of cytoplasmic effects on physiological traits.

Controlled laboratory assay using prepared stigmas on growth medium to test pollen germination and tube growth across species boundaries. Stigmas are prepared from unopened buds, treated with pure pol...

Replicated split-plot design with snowmelt manipulated at plot level and precipitation manipulated at subplot level.