Protocols

DNA extraction from hair samples followed by microsatellite genotyping to determine parentage relationships and construct molecular pedigrees for quantitative genetic analysis.

Progesterone concentrations measured using radioimmunoassay with tritiated progesterone, ether extraction, and antibody binding assay. Standard curves and duplicate samples used for quantification.

Fecal sample collection and zinc sulfate flotation protocol for detecting intestinal parasites including Ascaris, Eimeria, and Entamoeba in marmot feces.

RNA extraction from blood samples, library preparation, and high-throughput sequencing on Illumina platforms followed by read mapping and differential gene expression analysis using mixed models.