Protocols
9 protocols
Microsatellite genotyping (Polemoniaceae)
PCR amplification of microsatellite loci followed by capillary electrophoresis for allele sizing and polymorphism assessment.
Acrylic leaf imprint stomatal analysis
Acrylic imprints of leaf surfaces are made on glass slides and analyzed under compound microscope to count stomata and measure their diameters.
Fitness component quantification (Brassicaceae)
Comprehensive measurement of multiple fitness components including survival, reproductive success, fecundity, and growth rates to assess life history trade-offs under resource stress.
Hybrid zone cline analysis over elevational gradient (Polemoniaceae)
Long-term monitoring of plant morphological traits across permanent transects spanning a hybrid zone between two Ipomopsis species along an elevational gradient, with cline fitting to quantify trait g...
Ordinal rust infection severity assessment
Weekly assessment of fungal rust infection using a 6-class ordinal scale from absent (0) to 75-100% infected (5), with infection location recorded.
Pinene enantiomer scent manipulation (Polemoniaceae)
Controlled application of synthetic pinene enantiomers via cotton-filled microcentrifuge tube emitters to test effects of specific volatile compounds on insect oviposition behavior. Treatments applied...
diallel mating design (Polemoniaceae)
Production of F2 hybrid generations with alternate cytoplasmic backgrounds through controlled hand-pollination crosses. Allows testing of cytoplasmic effects on physiological traits.
negative binomial regression
Statistical analysis using zero-inflated negative binomial generalized linear mixed models to account for excess zeros in ecological count data, specifically pollen grain counts.
reflectance-based chlorophyll index (Polemoniaceae)
Non-destructive estimation of leaf chlorophyll concentration using reflectance spectroscopy and vegetation indices.